Effect of crude and partially purified epidermal mucus proteins of marine catfish Tachysurus dussumieri on human cancer cell line

Epidermal mucus protein of marine catfish Tachysurus dussumier was screened for its anticancer properties. The mucus sample was collected and categorized as crude and partially purified. Crude and partially purified samples consist of 0.48 ± 0.02 mg/mL and 0.82 ± 0.05 mg/mL proteins, 1.15 ± 0.06 mg/mL and 0.54 ± 0.02 mg/mL carbohydrates and 0.78 ± 0.04 mg/mL and 0.32 ± 0.03 mg/mL of lipids respectively. Cytotoxicity was expressed in the inhibition concentration (IC50) value 5 mg/mL at 72 h for crude sample and 3 mg/mL at 48 h for partially purified protein against human lung adenocarcinoma epithelial cell line (A549). DNA fragmentation revealed that the control cells has intact DNA, whereas cells treated with both the mucus proteins confirmed DNA fragments of different molecular range due to the induction of apoptosis.